England centre Ollie Lawrence appears unlikely to recover from a knee injury in time for Saturday's Guinness Six Nations clash with Italy in Rome.
Lawrence has been left out of a 36-man squad that will step up preparations for the Stadio Olimpico showdown but will continue his rehabilitation at England's training base.
The Bath player had an injection on the damaged joint last week but now looks set to miss the penultimate assignment of the Six Nations.
The same knee injury ruled him out of the first three rounds and he only made his comeback in last Saturday's 42-21 demolition by Ireland.
Tommy Freeman is the leading contender to replace him at outside centre in a positional shift from the wing, with Henry Slade also an option.
Forwards:
Ollie Chessum (Leicester Tigers)
Arthur Clark (Gloucester Rugby)
Alex Coles (Northampton Saints)
Luke Cowan-Dickie (Sale Sharks)
Chandler Cunningham-South (Harlequins)
Tom Curry (Sale Sharks)
Theo Dan (Saracens)
Trevor Davison (Northampton Saints)
Ben Earl (Saracens)
Ellis Genge (Bristol Bears)
Jamie George (Saracens)
Joe Heyes (Leicester Tigers)
Maro Itoje (Saracens)
Emmanuel Iyogun (Northampton Saints)
Jack Kenningham (Harlequins)
George Kloska (Bristol Bears)
Guy Pepper (Bath Rugby)
Henry Pollock (Northampton Saints)
Bevan Rodd (Sale Sharks)
Sam Underhill (Bath Rugby)
Backs:
Henry Arundell (Bath Rugby)
Seb Atkinson (Gloucester Rugby)
Elliot Daly (Saracens)
Fraser Dingwall (Northampton Saints)
George Ford (Sale Sharks)
Tommy Freeman (Northampton Saints)
George Furbank (Northampton Saints)
Cadan Murley (Harlequins)
Raffi Quirke (Sale Sharks)
Tom Roebuck (Sale Sharks)
Henry Slade (Exeter Chiefs)
Marcus Smith (Harlequins)
Fin Smith (Northampton Saints)
Ben Spencer (Bath Rugby)
Freddie Steward (Leicester Tigers)
Jack van Poortvliet (Leicester Tigers)
All times UK and Ireland
